HUMAN ANTIBODY POSSESSING BINDING ACTIVITY WITH MN AND NEUTRALIZATION OF CELLULAR ADHESION

摘要

FIELD: immunology, antibodies. ^ SUBSTANCE: invention elates to human monoclonal antibodies to MN and antibody fragments to MN that are targeted to repeat sequence GEEDLP within proteoglycan domain. Binding with a desired epitope is confirmed by competitive immunoenzyme analysis method ELISA wherein ELISA signal is attenuated in combined incubation with peptide comprising this repeat sequence (PGEEDLPGEEDLP). Binding inhibition can be confirmed by the Biacore study also wherein binding required antibodies with immobilized MN or proteoglycan peptides can be inhibited by peptide repeat sequence. In addition to binding with human peptide repeat sequence anti-MN can inhibit adhesion of CGL-1 cells to plastic plates covered by MN. Human antibodies anti-MN can be used in treatment of cancer diseases or for diagnosis of cancer diseases wherein the level of MN is increased. ^ EFFECT: valuable medicinal properties of antibodies. ^ 11 cl, 8 dwg, 2 tbl, 13 ex