| 摘要 |
FIELD: fish industry, in particular, artificial rearing of sturgeon fish. ^ SUBSTANCE: method involves diluting semen with freezing protective solution before freezing procedure, said solution containing saccharose, potassium chloride, and methanol; freezing in liquid nitrogen vapor; introducing into protective solution glycerin as additional freezing protective agent; freezing diluted semen in three stages; using protective solution components in the following ratio, wt%: saccharose 0.05-0.5; potassium chloride 0.01-0.15; methanol 6-12; glycerin 0.05-5; distilled water the balance. ^ EFFECT: retention of semen impregnation capacity after cryogenic preservation process owing to utilization of more effective composition of freezing protective solution and moderate freezing mode. ^ 2 cl, 3 tbl, 3 ex |
