METHOD FOR TREATING BIOMASS FOR PRODUCING CELL LYSATE CONTAINING PLASMID DNA

摘要

The invention relates to a method for the integrated treatment of biomass from a cell culture process for producing clear cell lysate containing plasmid DNA. According to the invention, in a first step filtering occurs in a filter in the presence of a filtering agent. In a second step, the biomass contained in the filter cake which is obtained is thermally decomposed and the cell lysate is filtered in another step. The obtained cell lysate containing plasmid DNA is characterised by a clarity of OD600 of at a maximum of 0.05E/cm and which is suitable for cloning, transformation, transfection, microinjection in cells, gene therapy, DNA vaccination and/or for polymerase chain reaction (PCR).