Analysis of cell for diagnosing cancer, comprises introducing cells on carrier, coloring the cells by first coloring means, creating and storing first digital image of the colored cells, and treating the cells with second coloring means

摘要

The analysis of cell for diagnosing cancer, comprises introducing cells to be analyzed on a carrier, coloring the cells by a first coloring means, creating and storing a first digital image of the colored cells, treating the cells on the carrier according to the reception of the first digital image with a second coloring means in such a manner that their optically measurable characteristics change, and creating and storing a second digital image of the cells introduced on the carrier. A group in preparations with the cells is colored by a coloring means of a highly sensitive analysis method. The analysis of cell for diagnosing cancer, comprises introducing cells to be analyzed on a carrier, coloring the cells by a first coloring means, creating and storing a first digital image of the colored cells, treating the cells on the carrier according to the reception of the first digital image with a second coloring means in such a manner that their optically measurable characteristics change, and creating and storing a second digital image of the cells introduced on the carrier. A group in preparations with the cells is colored by a coloring means of a highly sensitive analysis method and then the preparations with positive findings are further-treated. A selection is encountered from the cells and further analysis steps are concentrated on this selection. The selection on the cells takes a control function for further analysis steps. The group on the preparations is subjected to an automatic analysis step, which is used as pre-analysis for indicating suspicious cells. The digital image is produced with a linear scanner. The first- and the second digital image are brought to congruence, in that by the fusion of local image data a fine alignment is obtained. By the congruence, a larger area with lower resolution and a smaller area with higher resolution are gathered. The fine structuring of certain cell areas is analyzed on the basis of a multidimensional picture vector from the pixel values of different coloring. The object carrier is used with lattices or markers. The cells, which are separated during the coloring process from their position, are seized. A tissue area is analyzed by a histological investigation. Cell nucleus characteristics are used for classification of the cells. The cells from the histological preparation are distinguished with a computer-assisted scalpel. The cell is automatically conveyed in computer-controlled manner. A material is applied on the cells and its adhesive property is increased with a laser beam. By a position-controllable laser the coordinates before selected cells is illuminated. A data base-based workplace system is used in the analysis.