摘要 |
PROBLEM TO BE SOLVED: To provide a method for producing albumin having extremely low levels of or essentially free of colorants, metal ions, human proteins, host proteins, fragments of albumin, polymers or aggregates of albumin and viruses, essentially non-glycated, relatively high free thiol level and having complete C-terminal. SOLUTION: The process comprises passing albumin through positive mode cation exchange chromatography and then positive mode anion exchange chromatography. Other steps may also be employed, for example ultrafiltration, gel permeation chromatography, affinity chromatography binding the albumin and affinity chromatography binding contaminants. Elution of albumin from a material having no specific affinity for albumin with a compound having affinity for albumin is also disclosed. Ammonium ions are removed in this case by the use of counter ions. COPYRIGHT: (C)2008,JPO&INPIT
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