| 摘要 |
Disclosed herein is a process for the qualitative and quantitative determination of at least one in vitro amplified nucleic acid in a sealed reaction chamber, wherein subsequent to the amplification of the nucleic acid, at least one probe interacts with an at least partially denatured amplification product(s) in an optically detectable, differential manner, which varies as a function of decreasing temperature applied through a cooling gradient. This process allows the quantity and character of the amplification product(s) to be characterized. The entire amplification reaction, may be carried out in a sealed reaction chamber without intermittent opening, permitting an automated method of analyzing DNA and RNA amplification in qualitative and quantitative fashion on large series of samples. |
