RECOMBINANT INTERFERON-BETA WITH ENHANCED BIOLOGICAL ACTIVITY

摘要

Human interferon-ß protein analogs in which the asparagine at position 25 , numbered in accordance with native human interferon-ß, is recombinantly re placed with an aspartate residue exhibit a biological activity of human inte rferon-ß (e.g. IFN-ß 1b) at an increased level relative to IFN-ß 1b. These a nalogs are obtained by introducing a gene coding for Asp25 IFN-ß into a cell and expressing the recombinant protein. The resulting IFN-ß protein analog is suitable for large scale manufacturing for incorporation in HA-containing or HA-free therapeutics for treatment of diseases including multiple sclero sis. A reduced Lys endoproteinase-C peptide map technique that produces a fi ngerprint profile for proteins using an enzymatic digest followed by RP-HPLC is also useful in quality control as an ID test for the IFN-ß protein analo g products.