COMPETITIVE PARTICLE IMMUNOASSAY METHODS UTILIZING FLUORESCENCE MICROSCOPY

摘要

The present invention provides methods of measuring analytes in a sample by competitive immunoassay utilizing fluorescence microscopy. In particular embodiments, the invention provides a method of determining the gender of an avian embryo in ovo by determining the presence of an estrogenic steroid compound in a sample of embryonic fluid (e.g., allantoic fluid or blood) from the avian egg.