| 摘要 |
A method for immobilizing saccharide on the surface of a substrate is provided to immobilize directly the saccharide on the surface of a solid substrate without changing the structure of the saccharide and is applied to various saccharides. A method for immobilizing saccharide on the surface of a substrate such as gold comprises the steps of: (a) reacting an aldehyde of the saccharide selected from the group consisting of reducing monosaccharide, oligosaccharide having a terminal reducing sugar, and polysaccharide with an amine group of an amine compound represented by NH2-R-SH(where R is CC30-50 alkylene, C6-30 aryl or C6-30 aryl including C10-30 alkylene) to prepare a thiol group bound saccharide; and coating the thiol group bound saccharide to the surface of the substrate to bind the thio to the substrate surface, where when the saccharide is a monosaccharide, the reaction is done at a temperature of 20-90 deg.C for 0.5-2 hours, and when the saccharide is a polysaccharide, the reaction is done at a temperature of 30-90 deg.C for 0.5-2 hours. The method further comprises a step of reducing the thiol bound saccharide.
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