IDENTIFICATION AND CHARACTERIZATION OF ANALYTES FROM WHOLE BLOOD

摘要

The invention provides a method of preparing samples for improved detection of targets in whole blood by binding targets to multiple ligands. The method comprises providing ligands attached to a support, and contacting the ligands with targets in blood to allow at least one target to bind to at least one ligand. The method further comprises removal of non-bound targets and cellular components such as blood cells, platelets, abundant plasma proteins followed by dissociation and elution of the target(s). The eluted targets are detected by a variety of means in concentrations which are a function of their presence in one sample as compared with their concentration in a second sample and are simultaneously enriched for trace components.