METHOD OF DETECTING PROTEIN AND FLUORESCENT DYE TO BE USED THEREFOR

摘要

It is intended to provide a method of detecting protein at a high sensitivity with the use of a convenient procedure and a fluorescent dye to be used therefor. Namely, a detection method whereby a protein is detected by measuring a fluorescence based on a second fluorescence wavelength observed in the state of binding to the protein which is shorter than a first fluorescence wavelength observed in a free state. The fluorescent dye as described above is superior in heat stability to Cy3, Cy5 and Alexa dyes and shows no fluorescence elimination. Thus, it can be handled more easily and less expensively than Cy3 and Cy5 and, therefore, makes it possible to detect a protein at a lower cost.