| 摘要 |
PROBLEM TO BE SOLVED: To provide a cyanide-free lytic reagent composition and method for measuring the total hemoglobin concentration in a blood sample, for counting the number of leukocytes and for deferential counting of three leukocyte subpopulations including lymphocytes, monocytes, and granulocytes. SOLUTION: The cyanide-free lytic reagent composition includes: a hemolytic surfactant chosen from quaternary ammonium salts, pyridinium salts, organic phosphate esters, and alkyl sulfonates to lyse erythrocytes and release hemoglobin; and an organic ligand chosen from triazole and its derivatives, tetrazole and its derivatives, alkaline metal salts of oxonic acid, melamine, aniline-2-sulfonic acid, and the like to form a stable chromogen with hemoglobin. COPYRIGHT: (C)2008,JPO&INPIT |
