DETECTION OF PROTEASE AND PROTEASE ACTIVITY USING A SINGLE NANOCRESCENT SERS PROBE
摘要
This invention pertains to the in vitro detection of proteases using a single peptide-conjugate nanocrescent surface enhanced Raman scattering (SERS) probes with at least nanomolar sensitivity. The probe enables detection of proteolytic activity in extremely small volume and at low concentration. In certain embodiments the probes comprise an indicator for the detection of an active protease, where the indicator comprises a nanocrescent attached to a peptide, where said peptide comprises a recognition site for the protease and a Raman tag attached to the peptide.
申请公布号
WO2008018933(A2)
申请公布日期
2008.02.14
申请号
WO2007US10722
申请日期
2007.05.02
申请人
THE REGENTS OF THE UNIVERSITY OF CALIFORNIA;LIU, GANG, L.;ELLMAN, JONATHAN, A.;LEE, LUKE, P.;CHEN, FANQING, FRANK
发明人
LIU, GANG, L.;ELLMAN, JONATHAN, A.;LEE, LUKE, P.;CHEN, FANQING, FRANK