METHOD OF PRODUCING HETEROLOGOUS SHORT CHAIN POLYPEPTIDES BYYEAST WITH IMPAIRED YAP3 PROTEASE

摘要

The present invention relates to a novel method for the production of short chain polypeptides, including polypeptides having up to 3 disulfide bonds and/or structures rich in basic amino acid residues, and open structured short chain polypeptides, e.g. glucagon, glucagon like peptides and their functional analogues, in genetically modified yeast cells having reduced activity of YAP3 protease. The invention further comprises genetically modified yeast cells, and a method for the preparation of said yeast cells.