MAGNETICALLY ACTIVATED CELL SORTING FOR PRODUCTION OF PROTEINS

摘要

The invention includes methods of isolating DNA and a protein encoded thereb y, the protein being capable of binding to an antigen-bearing moiety. The methods comprise generating a DNA library expressing the protein in a virus vector. A magnetic label is added to cells expressing the antigen-bearing moiety and the cells are incubated with virus expressing the protein in the presence of an excess of unlabeled cells which do not express the antigen-bearing moiety to form a mixture, wherein the virus binds to the magnerically labeled cells. Cells having virus bound thereon are isolated from the mixtur e and DNA encoding the protein and the protein are obtained therefrom.