摘要 |
A culture method for high concentration of Thiocapsa roseopersicina is provided to maximize the growth of cells under photo-autotrophic conditions, so that a large quantity of hydrogenase is produced. A high concentration of Thiocapsa roseopersicina NCIB 8347 is photo-autotrophically cultured in a medium containing acetate as a carbon source under the carbon dioxide-containing nitrogen condition at 26 deg. C for 3-5 days, wherein the medium further contains ammonium chloride and a trace amount of elements including Na2S.9H2O, Na2.EDTA, FeSO4.7H2O, CoCl2.6H2O, MnCl2.2H2O, ZnCl2, NiCl2.6H2O, Na2MoO4.2H2O, H3BO3 and CuCl2.2H2O.
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