| 摘要 |
To provide a cell culturing method employing biomechanical stimulation loading and its system for applying, in in vitro culture, stimulation similar to vertical weight load stimulation or sideward shear stress stimulation acting on tissues in an aboveground living body as repeated biomechanical stimulation to culture cells, culture tissues or a construct including the culture cells. The system includes mechanical stimulation loading device ( 2 ) and culture receptacle ( 3 ) that are placed in carbon dioxide incubator ( 1 ), and control computer ( 4 ). Device ( 2 ) includes: weight loading piston ( 5 ); piston raising and lowering stage ( 21 ) supporting piston ( 5 ) to allow vertical movement of piston ( 5 ) within a designated range; and stage lifting and lowering mechanism ( 22 ) for vertically moving stage ( 21 ). Computer ( 4 ) drives mechanism ( 22 ) in a specified cycle to effect the vertical movement of stage ( 21 ). When stage ( 21 ) is lowered, culture (C) in receptacle ( 3 ) is weighted with piston ( 5 ).
|
