Methods for the preparation of immunoconjugates, in particular maytansinoids conjugated to a monoclonal antibody

摘要

Disclosed is a method for conjugating a maytansinoid to an antibody comprising; reacting a disulfide-containing linker with the antibody to form a modified antibody; removing unreacted linker from the modified antibody by tangential flow filtration; conjugating the modified antibody with the maytansinoid in a solvent comprising dimethylacetamide; and purifying the antibody via ion exchange chromatography.