A method for selecting cell lines to be used for nuclear transfer in mammalian species

摘要

Disclosed is a method for cloning a non-human mammal comprising: (i) selecting differentiated non-human mammalian cells to be used as a source of donor cells or donor nuclei according to their nuclear index and cleavage and/or fusion rates; (ii) obtaining at least one oocyte from a mammal of the same species as the source of donor cells or donor nuclei; (iii) enucleating said at least one oocyte; (iv) transferring a donor cell or donor cell nucleus into the enucleated oocyte to form a cell couplet; (v) simultaneously fusing and activating the cell couplet to form an embryo, wherein the cell couplet is activated after an initial electrical shock; (vi) culturing said embryo until greater than the 2-cell developmental stage; and (vii) transferring said embryo into a host mammal such that the embryo develops into a fetus.