发明名称 |
USES OF THE PSEUDOMONAS SYRINGAE EFFECTOR PROTEIN HopU1 RELATED TO ITS ABILITY TO ADP-RIBOSYLATE EUKARYOTIC RNA BINDING PROTEINS |
摘要 |
The bacterial plant pathogen Pseudomonas syringae injects effector proteins into host cells via a type III protein secretion system to cause disease. The invention relates to the discovery that the type III effector HopU1 is a mono-ADP-ribosyltransferase (ADP-RT) and suppresses plant innate immunity. The HopU1 substrates in Arabidopsis thaliana extracts were RNA-binding proteins that possess RNA-recognition motifs (RRMs). A. thaliana knock-out lines defective in the glycine-rich RNA-binding protein AtGRP7, a HopU1 substrate, were more susceptible than wild type plants to P. syringae. The ADP-ribosylation of AtGRP7 by HopU1 required two arginines within the RRM. The invention provides novel methods for the modulation of the innate immune response of a plant to a biotic stress, including methods for enhancing or suppressing the innate immune response of the plant.
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申请公布号 |
US2008028487(A1) |
申请公布日期 |
2008.01.31 |
申请号 |
US20070759054 |
申请日期 |
2007.06.06 |
申请人 |
THE UNIVERSITY OF NEBRASKA-LINCOLN |
发明人 |
ALFANO JAMES R.;FU ZHENG QING;ELTHON THOMAS E. |
分类号 |
A01H1/00;C12Q1/68 |
主分类号 |
A01H1/00 |
代理机构 |
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