| 摘要 |
A transgenic zebrafish for searching environmental contaminants and a method for preparing the same are provided to evaluate the preclinical effect and sensitivity by providing a biomarker for risk of the environmental contaminants. A method for preparing a transgenic zebrafish for searching environmental contaminants comprises the steps of: (a) cloning a pollutant response gene promoter of human such as HSRE(heat shock responsive element) and AhRE(aryl-hydrocarbon responsive element); (b) inserting the promoter into a vector expressing a fluorescent protein; and (c) introducing the vector into zebrafish. A transgenic zebrafish for searching environmental contaminants prepared by the method is characterized in that it radiates when it is exposed to environmental stress such as heat, cold, oxygen deficiency, Cd, L-azetidine-2-carboxylic acid, 2,3,7,8-tetrachlorodibenzo-p-dioxin, 3-methylcholanthrene, 3,4,5,3',4',5'-hexabromobiphenyl, Aroclor 1254 and benzo[a]pyrene.
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