DEVICES, METHODS AND APPLICATIONS FOR EXTRACTION OF MOLECULES FROM POLYMERIC GEL ELECTROPHORETIC MEDIA

摘要

A device and method to provide a simplified wash process and controlled disintegration of a soft substance, such as a gel, are disclosed. In use, a block of, e.g., gel matrix is placed in the device and washed with a series of appropriate solutions to remove interfering contaminants. The washing liquid is removed through a deformable narrow opening in the bottom of the device, and, subsequently, the gel block is extruded through the deformable narrow opening, by a physical force, such as centrifugal force, a vacuum or positive pressure from a gas or liquid, etc., resulting in fragmentation of the gel block into a series of particles of similar size. The fragmentation of the gel results from shear forces exerted onto the gel block traveling through the deformable narrow opening in the device. The rate of such fragmentation and resulting fragment size can be controlled by extruding the gel block using a predefined level of force as well as by controlling the dimensions and shape of the narrow opening (s) in the device.