| 摘要 |
Described herein are labeled probes and unlabeled oligonucleotides that a re useful for use in nucleic acid amplification reactions. These probes and oligonucleotides are modified to alter their sensitivity to primer-independe nt 5' exonuclease activity of a thermostable DNA polymerase relative to its corresponding unmodified counterpart. Non-symmetric polymerase chain reactio n (PCR) amplification and detection methods employing these labeled probes a nd unlabeled oligonucleotides are also described. Kits for nucleic acid ampl ification reactions including labeled probes and unlabeled oligonucleotides are also described.
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