EXPRESSION CASSETTE, USE OF THE EXPRESSION CASSETTE, VECTOR, HOST CELL, A METHOD FOR PRODUCING A POLYPEPTIDE

摘要

The subject matters of invention relate to expression cassette, use of the expression cassette, vector, host cell, a method for producing a polypeptide ensuring its stable expression by the prokaryotic host as well as an use of the expression cassette. The invention enables stable expression of the target polypeptide, in systems where DNA- dependent RNA polymerase recognises promoter regulating synthesis of target protein as well as selection marker, which is required for survival of the host.