NON-REPLICATING PARAMYXOVIRIDAE VIRUS VECTOR

摘要

A non-replicating SeV vector can be successfully produced which has the d eletion of all of genes for NP-protein, P-protein and L-protein (which are R NP-constituting proteins) in genomic RNA. It is confirmed that an NP/P/L-del eted SeV vector having a marker gene (e.g., GFP) integrated therein can achi eve high productivity, high foreign gene introduction efficiency and high fo reign gene expression efficiency (high MOI infection is required for achievi ng a high expression level). Since the vector has the deletion of L gene or NP, P and L genes, the quantity of a virus-derived protein expressed in a ho st cell can be decreased and therefore the immunogenicity upon in-vivo admin istration can be reduced.