CELL DEATH INDUCER

摘要

The object is to provide an antibody having a high cell death-inducing activity. To achieve the object, a mouse is immunized with a cell capable of expressing both human HLA class IA and human ß2 microglobulin (ß2M) to produce monoclonal antibodies. The monoclonal antibodies are screened for an antibody having a cell death-inducing activity, and 10 clones are obtained. The analysis of the clones reveals that three clones (C3B3, C11B9, C17D11 antibodies) which has an a2 domain of HLA class I antigen in their epitopes can cross-link with an anti-murine IgG antibody to show a potent cytotoxic activity. When a diabody of the C3B3 antibody is produced, it is found that the diabody shows a more potent anti-tumor effect compared to that of a diabody of a 2D7 antibody which is a known antibody against HLA class IA.