METHODS OF INTRODUCING TARGETED DIVERSITY INTO NUCLEIC ACID MOLECULES
摘要
Systems are disclosed that are useful for introducing one or more targeted positions or regions of diversity into a nucleic acid molecule. In certain embodiments, diversity in a targeted position or region is generated by providing one or more degenerate primer sets and a template nucleic acid molecule, wherein the primers are extended in opposite directions against the template nucleic acid molecule in a polymerase-mediated extension reaction. In certain embodiments, the generated nucleic acid molecule into which diversity has been introduced comprises single-stranded regions at its termini, which ingle-stranded regions are capable of annealing to each other.
申请公布号
WO2008008808(A2)
申请公布日期
2008.01.17
申请号
WO2007US73213
申请日期
2007.07.11
申请人
MODULAR GENETICS INC.;JARRELL, KEVIN;VISHWANATH, PRASHANTH;MCCARROLL, ROBERT;STOREK, MICHAEL
发明人
JARRELL, KEVIN;VISHWANATH, PRASHANTH;MCCARROLL, ROBERT;STOREK, MICHAEL