ONE STEP IMAC (MCAC) PURIFICATION OF PROTEINS

摘要

This invention relates to a process for isolation and/or purification of a protein of interest and to the use of IMAC as a capture and the main separation step. In one embodiment, the invention relates to a process for the isolation and/or purification of a protein of interest by use of immobilized metal affinity chromatography (IMAC), comprising the steps of (a) applying a mixture or solution comprising said protein to an IMAC matrix, (b) separating contaminant(s) by use of metal ion affinity of said protein to said IMAC matrix, under conditions suitable to keep said protein bound to the matrix, (c) separating the protein of interest from further contaminant(s) by use of both a non-metal ion affinity interaction and a metal ion affinity interaction between said protein and said IMAC matrix.