| 摘要 |
The antibiotic kanamycin is purified by passing an aqueous solution of kanamycin having a pH of 9.0 to 12.0 through a column of a highly porous strongly basic anion exchange resin in the hydroxyl form, collecting the effluent and isolating kanamycin therefrom. It is preferred that the column has a height of at least twice its diameter and contains at least one millilitre of wet resin per 100 ml. of the aqueous solution of kanamycin. The temperature is less than 140 DEG F. The resin is an insoluble, cross-linked capolymer of an aromatic monovinyl hydrocarbon and an aromatic divinyl hydrocarbon, the copolymer containing on the aromatic nuclei substituent groups having the general formula: <FORM:0871214/IV (b)/1> in which n is from 1 to 4 R1, R2 and R3 are monovalent hydrocarbon groups and Y is an anion, the number of the substituent groups being at least one for every 15 aromatic nuclei in the copolymer. Examples are Amberlites IRA-401, IRA-401S and IRA-411. (The word "Amberlite" is a Registered Trade Mark). The resin column is renovated by treatment with 1% aqueous sodium chloride. The kanamycin may be recovered from the effluent by adding H2SO4 to pH 10.5, extracting with n-butanol containing benzaldehyde, separating the butanol extract, treating with aqueous H2SO4 of pH 2.0, separating the aqueous phase, treating with carbon, filtering and precipitating the kanamycin by adding NH4OH to pH 8.2 and then methanol. Specification 817,385 is referred to. |
