摘要 |
An apparatus for ice seeding during cryopreservation of biological samples such as cells, harvested tissues, and cellular biological constructs such as culture tissue equivalents wherein ice seeding is perform ed by discharging a liquefied or chilled gas (60) from a nozzle (21) to the surface of a container (40) containing said biological sample in cryopreservative. Inside a freezing chamber (70), containers (40) are placed on racks (30) that are located proximal to a sprayrail (20) containing nozzles (21). Ice seeding is performed by discharge of liquefied or chilled gas (60) from the nozzles to the container surface a t the solid-liquid equilibrium temperature of the cryopreservative. Localized cooling of the container surface by the discharge results in ice seed formation within the container (40).
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