发明名称 METHODS FOR IDENTIFICATION OF ALLELES
摘要 <p>The invention provides a method for identification of alleles. In this method, genomic DNA is used as target. Multiple allele-specific PCR amplification are carried out with a group of primers comprising one or more allele-specific primers for a target gene, a universal primer, and a common primer; and a DNA polymerase without 5' to 3' exonuclease activity. The PCR products are hybridized with tag probes immobilized on a DNA chip. Results are determined based on the signal intensity and the position of the probe immobilized on the array. Each allele-specific primer comprises a unique tag sequence at the 5' end. Each tag probe immobilized on the DNA chip comprises a sequence identical to its corresponding tag sequence; and each tag probe hybridizes only with the complementary sequence in the PCR amplification product.</p>
申请公布号 WO2008003244(A1) 申请公布日期 2008.01.10
申请号 WO2007CN02039 申请日期 2007.06.29
申请人 CAPITALBIO CORPORATION;TSINGHUA UNIVERSITY;LI, CAIXIA;GAO, HUAFANG;LIU, XIANG;CAI, BIN;ZHANG, DI;CHENG, JING 发明人 LI, CAIXIA;GAO, HUAFANG;LIU, XIANG;CAI, BIN;ZHANG, DI;CHENG, JING
分类号 C12Q1/68 主分类号 C12Q1/68
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