| 摘要 |
PROBLEM TO BE SOLVED: To provide an observation system or a photometry system, and a method thereof, using an immersion substance capable of acquiring an image of excellent S/N ratio in very weak-fluorescence observation, and capable of facilitating optical regulation for total reflection illumination using an objective lens. SOLUTION: The fluorescence observation or fluorescence photometry system using the low-fluorescent immersion substance is constituted to make the low-fluorescent immersion substance satisfy the conditional expression B<SB>IM'</SB>/B<SB>IM</SB>≤0.7, and to make a refractive index nd get to 1.50 or more and less than and 1.70 in a d-ray (587.56 nm) in the low-fluorescent immersion substance, where B<SB>IM'</SB>is an average value of intensities of autofluorescence of the low-fluorescent immersion substance, and B<SB>IM</SB>is an average value of intensities of autofluorescence of an immersion substance used generally in the prior art. COPYRIGHT: (C)2008,JPO&INPIT
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