发明名称 |
MEASURING THROMBIN ACTIVITY IN WHOLE BLOOD |
摘要 |
<p>The invention relates to a method for in vitro determining thrombin activity in a sample wherein the sample is a blood sample and thrombin generation is measured by the steps of:
- contacting a layer of said sample with a fluorogenic substrate of thrombin, wherein said layer has a thickness within a range of 0.05 to 5 mm and a surface within a range of 10 to 500 mm 2 ;
- allowing thrombin to generate in said sample;
- measuring the fluorescence emitted from the surface of the layer, by the fluorescent group released from the fluorogenic substrate as a result of enzymatic action of generated thrombin on said fluorogenic substrate.</p> |
申请公布号 |
EP1875249(A1) |
申请公布日期 |
2008.01.09 |
申请号 |
EP20060753838 |
申请日期 |
2006.04.26 |
申请人 |
SYNAPSE B.V. |
发明人 |
HEMKER, HENDRIK, COENRAAD;BEGUIN SUZETTE;AL-DIERI RAED;WAGENVOORD ROBERT;NIJHUIS SABASTIAAN;GIESEN PETER |
分类号 |
G01N33/86;C12Q1/56 |
主分类号 |
G01N33/86 |
代理机构 |
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代理人 |
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主权项 |
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地址 |
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