AN ANTI-BACTERIAL PEPTIDE BASE PAIRS AND AN ANTI-BACTERIAL PEPTIDE ISOLATED FROM DUNG BEETLE, COPRIS TRIPARTITUS, LARVAE

摘要

A novel sequence and an antibacterial peptide isolated from larvae of Copris tripartitus are provided to be usefully used as a prophylactic and therapeutic agent of bacterial diseases of human and plant and show antibacterial activity against various pathogenic bacteria. A sequence of an antibacterial peptide isolated from larvae of Copris tripartitus(SEQ ID : NO. 3) is represented by the following : AACGGTTCTG AATTCTTAAC CAGTTCATAC CCAGCTATAA TTTGAATTAC 50 GTCTTTTAAA AAGATGGCAA AATTAATTGC TTTTGCATTA GTAGCTTCGT 100 TATGCCTGTC TATGGTGTTG TGCAATCCGT TACCAGAAGA AGTCCAAGAA 150 GAGGGACTTG TCAGACAAAA AAGGGTAACC TGTGACGTGT TAAGTTTCGA 200 AGCCAAAGGA ATAGCTGTAA ATCATTCGGC TTGTGCGCTA CATTGTATTG 250 CCTTACGTAA AAAAGGTGGT TCTTGTCAAA ATGGTGTGTG TGTTTGTAGA 300 AATTAATTAA CAAGCTGATT GTCCTTTTTT TTAATTGTGG CCGGATGATA 350 TTATTTTCAG TGTAATAAGA ACAAAAATAA AGATTTATAA CTATTAAAAA 400 AAAAAAAAAA AA 412. A novel antibacterial peptide(SEQ ID : NO. 4) is encoded by the sequence and consists of an amino acid sequence of Met Ala Lys Leu Ile Ala Phe Ala Leu Val Ala Ser Leu Cys Leu 15 Ser Met Val Leu Cys Asn Pro Leu Pro Glu Glu Val Gln Glu Glu 30 Gly Leu Val Arg Gln Lys Arg Val Thr Cys Asp Val Leu Ser Phe 45 Glu Ala Lys Gly Ile Ala Val Asn His Ser Ala Cys Ala Leu His 60 Cys Ile Ala Leu Arg Lys Lys Gly Gly Ser Cys Gln Asn Gly Val 75 Cys Val Cys Arg Asn 80.