发明名称 |
Methods and compositions for identifying morphogen analogs |
摘要 |
Disclosed herein are methods and compositions for identifying morphogen analogs. The preferred methods and compositions relate to the discovery that morphogen upregulation of the mouse type X collagen promoter activity is mediated by a MEF-2 like sequence and requires an adjacent AP-1 sequence. Certain methods rest on the use of test cells comprising DNA defining a morphogen-responsive transcription activating element operatively associated with a reporter gene. Other methods rest on the use of DNAs for measuring morphogen-inducible DNA-binding. In certain preferred embodiments, the methods and DNAs involve an osteogenic protein 1 (OP-1) responsive transcription activating element. Substances that mediate interaction with and/or activate the OP-1 responsive transcription activating element are considered herein likely to be useful for reproducing in vivo effects of morphogens such as OP-1.
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申请公布号 |
US2008003675(A1) |
申请公布日期 |
2008.01.03 |
申请号 |
US20070801900 |
申请日期 |
2007.05.11 |
申请人 |
CURIS, INC. |
发明人 |
HARADA SHUN-ICHI;RODAN GIDEON A.;SAMPATH KUBER T. |
分类号 |
C12N5/10;C12N15/09;A61K31/00;A61K38/00;A61K38/27;A61K45/00;A61P1/00;A61P1/04;A61P1/16;A61P9/00;A61P9/10;A61P19/00;A61P19/08;A61P25/28;A61P29/00;A61P43/00;C07H21/04;C07K14/51;C12Q1/68 |
主分类号 |
C12N5/10 |
代理机构 |
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代理人 |
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主权项 |
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地址 |
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