| 摘要 |
The present invention relates to methods for the identification of an antibody that is specific to an epitope of interest. In certain preferred embodiments, methods of the invention comprise an enrichment procedure and/or a subtractive selection that facilitates the rapid identification of an epitope specific antibody. Methods of the invention also facilitate the identification of an antibody derived from a desired species that is specific for the epitope recognized by another antibody derived from a different species. Methods of the invention further facilitate the identification of an antibody specific to an epitope found in two different antigens, and an antibody capable of binding an epitope of interest but not a homologue of the epitope. Methods of the invention also facilitate the identification of an antibody specific for an antigen presented in one sample, but not, or significantly less, in another sample. |
