| 摘要 |
Genetically engineered, CE7-specific redirected immune cells expressing a cell surface protein having an extracellular domain comprising a receptor which is specific for CE7, an intracellular signaling domain, and a transmembrane domain, and methods of use for such cells for cellular immunotherapy of CE7+ neuroblastoma are disclosed. In one embodiment, the immune cell is a T cell and the cell surface protein is a single chain FvFc:zeta receptor where Fv designates the V<SUB>H </SUB>and V<SUB>L </SUB>chains of a single chain monoclonal antibody to CE7 linked by peptide, Fc represents a hinge-C<SUB>H</SUB>2-C<SUB>H</SUB>3 region of a human IgG<SUB>1</SUB>, and zeta represents the intracellular signaling domain of the zeta chain of human CD3. DNA constructs encoding a chimeric T-cell receptor and a method of making a redirected T cell expressing a chimeric T cell receptor by electroporation using naked DNA encoding the receptor are also disclosed.
|
