A METHOD FOR PURIFICATION GLYCOSAMINOGLYCANS FROM ASCIDIAN TUNIC

摘要

A method for preparing a glycosaminoglycan from ascidian tunic is provided to obtain the glycosaminoglycan with high yield and high purity more economically by removing a low molecular weight protein and a pigment compound using an ion exchange resin. A method for preparing a highly pure glycosaminoglycan comprises the steps of: (a) crushing ascidian tunic preserved at a temperature of -40 deg.C and steaming the crushed tunic at a temperature of 105-121.1 deg.C for 1-2 hours; (b) after adding 1.0-5.0 wt.% of flavourzyme 500 MG to the steamed tunic, treating it with a buffer solution with the pH of 5.0-8.0 at a temperature of 40-65 deg.C for 4-6 hours to decompose enzymatically; (c) after centrifuging the enzymatically decomposed tunic with the speed of 6,000-8,000 rpm, concentrating a filtered supernatant using a vacuum evaporator at a temperature of 30-60 deg.C into Brix of 8.0-15.0; (d) after centrifuging the concentrate solution and adjusting the pH of an obtained supernatant into 5.5-7.5, putting it in a cation exchange resin column and eluting it to purify; and (e) after adding alcohol to a filtered solution obtained from the step(d) to precipitate and centrifuging it, vacuum freeze-drying the precipitate.