| 摘要 |
<p>The present teachings provide methods, compositions, and kits for amplifying nucleic acids. The amplified nucleic acids can then be sequenced. The sequencing reaction for such amplified nucleic acids can provide additional sequence information, and less redundancy, as compared to conventional approaches. In some embodiments, a target polynucleotide is amplified with a primer to form an amplicon comprising a type IIs restriction enzyme recognition site. Following digestion with a type IIs restriction enzyme, and ligation of an adapter, a sequencing primer can be hybridized to the adapter, and a sequencing reaction performed. The sequencing reaction results in the omission of unwanted sequence information that was present in the amplification primer.</p> |
