| 摘要 |
An in vivo method for the construction of randomized gene libraries and/or domain replacement in gene libraries by homologous recombination using a Kluyveromyces lactis killer toxin, in particular the (gamma-subunit of the K. lactis killer toxin, as negative selection marker is described. The use of the (gamma-subunit of K. lactis as negative selectable marker increases the percentage of randomized clones.
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