<p>The invention relates to a valve assembly (20) for use in an access port, in particular a medical access port. The valve assembly comprises a first sealing valve (1 ) and a second sealing valve (2). The second sealing valve (2) is provided with an annular base (21) and a central member (23) with a central bore (24). The central member (23) is at the outer circumference movably suspended to the annular base (21) by means of a flexible diaphragm (22), which diaphragm (22) is more flexible than the central member (23). The invention also relates to a medical access port provided with such a valve assembly.</p>