PRODUCTION PROCESS FOR PLANT BIOMASS AND ITS USE

摘要

The present invention relates to a production process for plant biomass and the subsequent use thereof in the production of substances of industrial interest, in particular biologically active substances. More in particular, the present invention relates to a method for the cultivation of a plant cell line capable of producing metabolites of interest, comprising a selection phase for the cell line which includes the following cycle of operations: a) cultivating on solid medium a predetermined stabilized plant cell line for a time sufficient to obtain the multiplication of said cell line to give substantially distinct cellular clusters; b) taking from said solid medium said substantially distinct cellular clusters and placing each of them into a distinct liquid culture medium; c) cultivating each of said substantially distinct cellular clusters in said liquid culture medium for a time sufficiently long to allow the multiplication of said cellular clusters and the analytical determination of the primary and/or secondary metabolites produced by it; d) operating a qualitative and quantitative determination of the primary and/or secondary metabolites produced by each of said cellular clusters in said liquid culture medium; e) selecting the cellular cluster capable of producing the greatest quantity of said metabolite of interest; f) repeating the cycle of operations according to steps a), b), c), d) and e) on said cellular cluster selected according to step e) for a sufficient number of times until the quantity of said metabolite of interest produced by a selected cellular cluster and by the cellular cluster derived from a successive cycle of the selection operation remains substantially unchanged.