SINGLE-MOLECULE PCR ON MICROPARTICLES IN WATER-IN-OIL EMULSIONS
摘要
Modulation of the viscosity of the oil phase of a microemulsion used for amplification of DNA on a bead increases the homogeneity of product beads and the amount of amplified DNA per bead. Moreover the number of separate microemulsion populations that can be formed in parallel is increased using multi-well plates and mixer mill disruptor machines designed to lyse biological samples.
申请公布号
WO2007149432(A2)
申请公布日期
2007.12.27
申请号
WO2007US14273
申请日期
2007.06.19
申请人
THE JOHNS HOPKINS UNIVERSITY;DIEHL, FRANK;KINZLER, KENNETH, W.;VOGELSTEIN, BERT