发明名称 |
Insulin-secreting immortalized liver cell line modified by glucose sensitivity |
摘要 |
The present invention provides a cell line which can be substituted for beta cells in human mature pancreatic islets and express insulin in a glucose-concentration dependent manner, and enables the easy obtainment of the number of cells which meets the demand. The present invention also provides a therapeutical cell preparation for treating diabetes. The cell lines of the present invention can be obtained by integrating both a nucleotide sequence encoding tamoxifen-induced Cre recombinase and a nucleotide sequence encoding insulin regulated by glucose-sensitive promoter into the chromosome in a human immortalized hepatic cell line FERM BP-7498 containing the TERT gene inserted in between a pair of LoxP sequences.
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申请公布号 |
US7312077(B2) |
申请公布日期 |
2007.12.25 |
申请号 |
US20030432592 |
申请日期 |
2003.06.02 |
申请人 |
KOBAYASHI NAOYA;TANAKA NORIAKI;OKITSU TERU;YOON JI-WON;JUN HEE-SOOK;SHIN SEUNGJIN |
发明人 |
KOBAYASHI NAOYA;TANAKA NORIAKI;OKITSU TERU;YOON JI-WON;JUN HEE-SOOK;SHIN SEUNGJIN |
分类号 |
C12N5/00;A01N63/00;A61K35/12;A61K35/407;A61K48/00;A61P3/10;C07K14/62;C12N5/02;C12N5/071;C12N5/10;C12N15/09;C12N15/63;C12N15/90 |
主分类号 |
C12N5/00 |
代理机构 |
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地址 |
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