摘要 |
The present invention provides systems and methods for large-scale genetic measurements by generating from a sample labeled target sequences whose length, orientation, label, and degree of overlap and complementarity are tailored to corresponding end-attached probes of a solid support so that signal-to-noise ratios of measurement from specifically hybridized labeled target sequences are maximized. Systems for implementing methods of the invention include a set of sample-interacting probes to produce amplicons that either each contain a segment of a target polynucleotide or an oligonucleotide tag that corresponds to a segment of a target polynucleotide, one or more solid phase supports that contain a plurality of end-attached probes, and methods of generating from sample-interacting probe amplicons from which labeled target sequences are tailored for hybridization to the solid phase supports, such as microarrays. In one aspect, labeled target sequences and end-attached probe of the solid phase supports comprise oligonucleotide tags and tag complements, respectively, selected from a minimally cross-hybridizing set. |
申请人 |
PARALLELE BIOSCIENCES, INC.;NAMSARAEV, EUGENI;KARLIN-NEUMANN, GEORGE;FAHAM, MALEK;JAIN, MANEESH;HARDENBOL, PAUL;WILLIS, THOMAS, D.;WANG, ZHIYONG |
发明人 |
NAMSARAEV, EUGENI;KARLIN-NEUMANN, GEORGE;FAHAM, MALEK;JAIN, MANEESH;HARDENBOL, PAUL;WILLIS, THOMAS, D.;WANG, ZHIYONG |