CONTROL OF THE FORMATION OF DOUBLE-STRANDED DNA

摘要

Disclosed is a method for increasing a melting temperature (Tm) of double-stranded DNA. Also disclosed is a reagent for use in the method. More specifically, disclosed is an agent for increasing a melting temperature (Tm) of a DNA molecule having a G-G sequence composed of two contiguous guanine (G) residues and also having a G-G mismatch and at least one other mismatch, which comprises a naphthyridine carbamate dimer derivative represented by the general formula (1): (1) wherein R<SUP>1</SUP> and R<SUP>2</SUP> independently represent a hydrogen atom or a group such as a hydrocarbon group; R represents an alkylene group; X represents an oxygen or sulfur atom; and R<SUP>3</SUP> represents a hydrogen atom, an alkyl group having 1 to 5 carbon atoms, or a group represented by the general formula (2): (2) wherein R<SUP>1</SUP> and R<SUP>2</SUP> independently represent a hydrogen atom or a group such as a hydrocarbon group; R represents an alkylene group; X represents an oxygen or sulfur atom; and L represents a linker group for binding two nitrogen atoms together. Also disclosed is a method using the agent.