OPTICAL APPARATUS FOR SIMULTANEOUSLY MEASURING THE SCATTERING AND CONCENTRATION OF SIGNALS OF MACROMOLECULES IN A FLOW CELL

摘要

This invention relates to a fiber optic apparatus for simultaneously measuring the scattering and concentration signals of macromolecules in a flow cell 3 . The apparatus is based on the delivery/focusing of both a laser and ultraviolet light source to the same physical position in a low volume flow cell 4 , via a bifurcated optical fiber 3 . This configuration allows the light scattering and concentration signal changes associated with a macromolecular solution passing through the flow channel to be measured simultaneously. This invention also relates to a method that uses the optical apparatus 10 to determine properties of a macromolecular solution such as the ideal crystallization and/or formulation conditions (via B₂₂) for a given protein solution.