METHOD AND SET FOR ENZYME IMMUNOASSAY OF EFFECT OF SUBSTANCES ON BINDING C1q COMPLEMENT SUBCOMPONENTS

摘要

FIELD: medicine, medicinal immunology. ^ SUBSTANCE: method involves sorption C1q complement subcomponent into microplate wells followed by addition of analyzed substance in the chosen concentrations and a conjugate of immunoglobulin G with enzyme. Then method involves determination of bound IgG by the end product of enzymatic reaction followed by comparing data about the amount of bound IgG in the presence of the definite concentration of analyzed substance and in its absence, and the inhibition constant is determined for this substance in binding C1q subcomponent with IgG. The set for enzyme immunoassay of effect of substances on binding C1q complement subcomponents comprises microplate with sorbed C1q complement subcomponent, conjugate of enzyme with nonspecific human immunoglobulin G and substrate-containing buffer. Using the proposed method provides assaying the effect of substances on binding C1q complement subcomponents for a single step and excluding necessity for using specific antibodies. ^ EFFECT: improved method for assay. ^ 2 ex