| 摘要 |
FIELD: veterinary science. ^ SUBSTANCE: the present innovation deals with collecting drone bees' larvae during preimaginal phase into a sterile vial. Then they should be poured with 40%-ethanol at the ratio of 1 portion of alcohol and 9 portions of drone bees' larvae to be frozen for about 23.5-24 h and defrosted for 5-10 min at (+38; +40)°C and homogenized. Homogenizate should be treated with ultrasound at maximal power of 400 W, current of 0.7 A and frequency of 35 kHz at exposure being 5 min to be then supplemented with stabilizing medium at the ratio of 1 portion of homogenizate suspension and 9 portions of 5%-glucose solution and rifampicin at the ratio of 30 mg/l homogenizate suspension. Then the preparation obtained should be packed into sterile vials followed by freeze drying. Moreover, freeze drying should be fulfilled at preliminary freezing at (-40; -45°)C for about 12-14 h followed by freeze drying at creating vacuum of 5-8 mV, not less and heating up to (+44; +45)°C. To finish drying one should fulfill this procedure at preparation's temperature being (+22; +24)°C. The innovation enables to obtain high-efficiency preparation being of no allergic action and prolonged terms of storage. ^ EFFECT: higher efficiency. ^ 1 cl, 5 ex, 1 tbl |
