DETECTING METHOD FOR CARBAMATE BASED AND ORGANIC PHOSPHATE BASED AGRICULTURAL CHEMICALS

摘要

A method for detecting carbamate-based and organic phosphate-based agricultural chemicals is provided to secure excellent detection sensitivity and shorten the detection time, thereby significantly improving the detecting efficiency. A method for detecting carbamate-based and organic phosphate-based agricultural chemicals comprises the steps of: (a) activating the surface of a metallic thin film chip by allowing N-hydroxysuccinimide and [N-ethyl-N'(dimethylaminopropyl)carbodiimide] to flow on the surface thereof; (b) diluting an antibody using a pH buffer solution with a pH of 3.5-5.0 to prepare a diluted solution of the antibody; (c) allowing the diluted solution to flow on the surface of the chip to fix the antibody on the surface of the chip; (d) providing an amine-based compound to the surface to stabilize the antibody; (e) providing a sample to be detected to the antibody fixed metallic thin film chip; (f) analyzing the sample; and (g) dissociating the sample from the metallic thin film chip, wherein the metallic thin film chip is made of Au and the antibody is glutathione s-transferase(GST) or acetylcholinesterase(ACHE).